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t7 or sp6 rna polymerases  (Promega)

 
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    Structured Review

    Promega t7 or sp6 rna polymerases
    T7 Or Sp6 Rna Polymerases, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t7+and+sp6+rna+polymerases/sp6+rna+polymerase/pm40398074-331-37-55
    Average 90 stars, based on 1 article reviews
    t7 or sp6 rna polymerases - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Osteoblast function is compromised at sites of focal bone erosion in inflammatory arthritis.
    Article Snippet: In rheumatoid arthritis (RA), synovial inflammation results in focal erosion of articular bone.. Despite treatment attenuating inflammation, repair of erosions with adequate formation of new bone is uncommon in RA, suggesting that bone formation may be compromised at these sites.. Dynamic bone histomorphometry was used in a murine model of RA to determine the impact of inflammation on osteoblast function within eroded arthritic bone.

    Article Title: Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.
    Article Snippet: Characterized by ankyrin repeat motifs, the feminization-1 (fem-1) gene plays an essential role in sex determination/differentiation in Caenorhabditis elegans.. However, there are only a few reports on fem-1 in crustaceans.. In this study, a fem-1 gene (Mrfem-1) was first isolated from the giant freshwater prawn Macrobrachium rosenbergii.

    Article Title: In Situ Localization and Rhythmic Expression of Ghrelin and ghs-r1 Ghrelin Receptor in the Brain and Gastrointestinal Tract of Goldfish ( Carassius auratus )
    Article Snippet: .. Antisense and sense mRNA probes were obtained with DIG RNA labeling MIX (Roche Diagnostic, Mannheim, Germany) by in vitro transcription with T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for preproghrelin gene, and with T7 and T3 RNA polymerases (Promega, Madison, WI, USA) for ghs-r1a gene probes. ..

    Synthesized:

    Article Title: Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.
    Article Snippet: Characterized by ankyrin repeat motifs, the feminization-1 (fem-1) gene plays an essential role in sex determination/differentiation in Caenorhabditis elegans.. However, there are only a few reports on fem-1 in crustaceans.. In this study, a fem-1 gene (Mrfem-1) was first isolated from the giant freshwater prawn Macrobrachium rosenbergii.

    Article Title: Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey
    Article Snippet: .. Caja-G plasmid DNA was linearized and riboprobes were synthesized using T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (Hartmann Analytic GmbH, Braunschweig, Germany; specific activity, 3,000 Ci/mmol) for 1 h at 37°C. .. Probes were purified using Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionized formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1 × Denhardt's solution) was added to yield a final probe activity of 5 × 10 4 cpm.

    Article Title: Expression of the Axonal Membrane Glycoprotein M6a Is Regulated by Chronic Stress
    Article Snippet: .. M6a plasmid DNA was linearized and riboprobes were synthesized with T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (ICN; specific activity 3000 Ci/mmol) for 1 h at 37°C. .. Probes were purified with Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionised formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, ph 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1× Denhardt's solution) was added to give a final probe activity of 5×10 4 CPM.

    In Vitro:

    Article Title: Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.
    Article Snippet: Characterized by ankyrin repeat motifs, the feminization-1 (fem-1) gene plays an essential role in sex determination/differentiation in Caenorhabditis elegans.. However, there are only a few reports on fem-1 in crustaceans.. In this study, a fem-1 gene (Mrfem-1) was first isolated from the giant freshwater prawn Macrobrachium rosenbergii.

    Article Title: In Situ Localization and Rhythmic Expression of Ghrelin and ghs-r1 Ghrelin Receptor in the Brain and Gastrointestinal Tract of Goldfish ( Carassius auratus )
    Article Snippet: .. Antisense and sense mRNA probes were obtained with DIG RNA labeling MIX (Roche Diagnostic, Mannheim, Germany) by in vitro transcription with T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for preproghrelin gene, and with T7 and T3 RNA polymerases (Promega, Madison, WI, USA) for ghs-r1a gene probes. ..

    Article Title: Extensive human cytomegalovirus (HCMV) genomic DNA in the renal tubular epithelium early after renal transplantation: Relationship with HCMV DNAemia and long-term graft function.
    Article Snippet: Ying Ting Li, Vincent C. Emery,* Saloni Surah, Michael Jarmulowicz, Paul Sweny, I. Michael Kidd, Paul D. Griffiths, and Duncan A. Clark Department of Infection, University College Medical School (Campus), Rowland Hill Street, Hampstead, London, United Kingdom Department of Nephrology and Transplantation, University College Medical School (Campus), Rowland Hill Street, Hampstead, London, United Kingdom Department of Histopathology, University College Medical School (Campus), Rowland Hill Street, Hampstead, London, United Kingdom

    Article Title: Dynamic Hedgehog signalling pathway activity in germline stem cells
    Article Snippet: .. Briefly, sense and antisense DIG-labelled cRNA probes were synthesized by in vitro transcription from plasmid cDNA with digoxigenin-UTP (Roche Diagnostics) using T7 and SP6 RNA polymerases (Promega) and quantified by dot blot according to the DIG-UTP manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey
    Article Snippet: .. Caja-G plasmid DNA was linearized and riboprobes were synthesized using T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (Hartmann Analytic GmbH, Braunschweig, Germany; specific activity, 3,000 Ci/mmol) for 1 h at 37°C. .. Probes were purified using Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionized formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1 × Denhardt's solution) was added to yield a final probe activity of 5 × 10 4 cpm.

    Article Title: Expression of the Axonal Membrane Glycoprotein M6a Is Regulated by Chronic Stress
    Article Snippet: .. M6a plasmid DNA was linearized and riboprobes were synthesized with T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (ICN; specific activity 3000 Ci/mmol) for 1 h at 37°C. .. Probes were purified with Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionised formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, ph 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1× Denhardt's solution) was added to give a final probe activity of 5×10 4 CPM.

    Activity Assay:

    Article Title: Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey
    Article Snippet: .. Caja-G plasmid DNA was linearized and riboprobes were synthesized using T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (Hartmann Analytic GmbH, Braunschweig, Germany; specific activity, 3,000 Ci/mmol) for 1 h at 37°C. .. Probes were purified using Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionized formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1 × Denhardt's solution) was added to yield a final probe activity of 5 × 10 4 cpm.

    Article Title: Expression of the Axonal Membrane Glycoprotein M6a Is Regulated by Chronic Stress
    Article Snippet: .. M6a plasmid DNA was linearized and riboprobes were synthesized with T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33 P-UTP (ICN; specific activity 3000 Ci/mmol) for 1 h at 37°C. .. Probes were purified with Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionised formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, ph 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1× Denhardt's solution) was added to give a final probe activity of 5×10 4 CPM.

    Dot Blot:

    Article Title: Dynamic Hedgehog signalling pathway activity in germline stem cells
    Article Snippet: .. Briefly, sense and antisense DIG-labelled cRNA probes were synthesized by in vitro transcription from plasmid cDNA with digoxigenin-UTP (Roche Diagnostics) using T7 and SP6 RNA polymerases (Promega) and quantified by dot blot according to the DIG-UTP manufacturer’s instructions. ..



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